in vitro Production
At Eurogentec, monoclonal antibodies are produced exclusively in vitro, in accordance with the Belgian animal regulations and the 2010-63-EU directive for animal welfare.
Up to 100g mAb
Our facilities are equipped to produce from mg to 100g mAb.
For requests of 200mg and more, a test study is systematically performed to evaluate the clone productivity.
The Best production scheme
Based on the requested mAb amount and the clone productivity, we select the best production scheme using flasks, roller bottles or bioreactors.
Mycoplasma diagnostics and cell line decontamination can also be performed.
Boost my hybridoma’s
Our antibody experts have established a proprietary protocol to boost the productivity of hybridomas. It usually gives a productivity increase from +20 to +600% (recorded so far).
Figure 1 : Evaluation of our optimized protocol on the relative antibody production of 8 randomly selected hybridomas, including both IgG and IgM.
Figure 2 : Reproducibility of our optimized protocol on the antibody production of two randomly selected hybridomas.
Upgrade your antibody
By mixed-mode chromatography.
Affinity chromatography (protein A,G,L), ion exchange, gel filtration chromatography, IgM purification.
Solutions for DNA and endotoxins removal.
Physico Chemical QCs :
Purity, Quantity, Integrity, Sterility,…
Technical QCs :
Method validation (ELISA, FACS, IHC,…)
Functional QCs :
Binding, cytotoxicity, antibody-dependent cell-mediated cytotoxicity (ADCC), CDC, in vivo testing….
Isotyping, sequencing, serum-free production, cell-line adaptation, cell banking, productivity assessment, variable chains sequencing, cloning for recombinant antibody generation.
ELISA tests can be developed.