At Eurogentec we produce custom Llama antibodies and offer VHH production in collaboration with QVQ.
The production process and Options are detailed here under.
At Eurogentec, llama or alpaca immunizations are conducted using customized protocols and antigens to produce heavy chain only antibodies.
Serum samples are available for testing or for IgG’s isolation.
5 – 10 weeks*
The program can be stopped at this step and you receive unpurified pAb (blood or serum).
& mRNA extraction
Blood lymphocytes necessary for VHH production are poorly stable.
So we provide the service for lymphocyte isolation from a blood sample (+/- 250 mL) and the RNA extraction.
The program can be stopped at this step and you receive mRNA.
At QVQ, VHH phage display libraries are generated to select and screen for high affinity monoclonal VHH.
Step 1: Two libraries with size >107 different clones and > 90% insert.
Step 2: The sequences of at least 6 binders from 2 families.
Step 3: 0.5 mg protein of the lead clones produced in E.coli and purified (>90%).
VHH fragments can be custom labeled for a wide range of applications including immunofluorescence, FACS analysis, in vivo imaging, PET imaging, MRI imaging, IHC and electron microscopy.
Large scale and GMP production
We can produce large quantities of VHH antibody fragment in yeast for animal experiments or crystallography up to GMP grade for microdosing studies in human.
The sequence of the VHH fragments can be optimized for production, physical and “in product” stability and various types of clinical use.
VHH antibody fragments can be generated in bivalent, bispecific or custom made formats.
Anti-llama secondary antibodies
Immunoglobulins G from camelids are a mixture of IgG1, IgG2 and IgG3. Only IgG2 and IgG3 are heavy-chain antibodies.
To distinguish IgG2/3 from IgG1, we provide unlabeled and labeled mouse anti-llama IgG1 and mouse anti-llama IgG2/3 secondary antibodies.
We also offer a polyclonal anti-llama VHH allowing for the detection of a very large panel of VHHs from different framework sequence families.
Interested in anti-llama antibodies ?
Anti-IgG1 and anti-IgG2/3 are available unconjugated, or conjugated to biotin or R-PE. Anti-VHH is available as a non-conjugated Ab.
Should you be interested in any other conjugation, please contact us.