Custom monoclonal antibodies
hybridoma development

We generate murine hybridomas
from a large panel of antigens using a stepwise approach.

A reporting is sent after each phase and discussed with the customer for potential adaptation. If no positive hybridoma is obtained after phase 3, only phase 1 will be charged.

Set-up

1. Project discussion

Selection of the best strategy

2. Antigen design and production

Various antigens can be used:

  • peptides
  • proteins
  • bacteria
  • inactivated viruses
  • transfected cells
  • small molecules

Hybridoma Development

1. Immunization

The success highly depends on the antigen quality. Several mice are immunized to allow the selection of the best responder

2. Fusion

Using the GentleMACS and optimizing the myeloma bank management makes this fusion step a very reproducible process

3. Hybridoma screening & selection

The screening steps can be performed using several technologies, including ELISA, FACS, IF and IHC

4. Cloning, isotyping and cell banking

Benefit from our personalized project
proposals and expertise.